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Tissue factor expression and angiogenesis in human glioma
1Department of Laboratory Medicine, Hua Shan Hospital, Fudan University, Shanghai, PR China.
Clinical Biochemistry
|July 24, 2002
Summary
Tissue factor (TF) is highly expressed in gliomas, correlating with malignancy grade and vascularity. This suggests TF plays a role in glioma angiogenesis and tumor progression.
Area of Science:
- Oncology
- Vascular Biology
- Molecular Biology
Background:
- Tissue factor (TF) initiates coagulation and is expressed in tumors.
- TF expression is a marker for malignant angiogenesis in solid tumors.
- The role of TF in glioma angiogenesis and progression is not well understood.
Purpose of the Study:
- To investigate TF expression in human gliomas.
- To determine the relationship between TF expression, glioma grade, and tumor vascularity.
Main Methods:
- Quantitative reverse transcription PCR and immunofluorescence were used.
- Analysis was performed on glioma cell lines, normal brain tissue, and 34 glioma surgical specimens (grades I-IV).
- Microvessel density was assessed using von Willebrand factor staining.
Main Results:
- TF was not detected in normal brain tissue.
- TF expression increased with glioma grade, present in 20% of grade I and 90% of grade IV gliomas.
- TF mRNA levels were significantly higher in gliomas than normal brain tissue and correlated with vascularity.
Conclusions:
- TF is expressed in gliomas.
- TF expression correlates with glioma histologic grade and vascularity.
- These findings support a role for TF in glioma angiogenesis.