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Updated: Jul 20, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
Published on: November 18, 2014
Scotin, a novel p53-inducible proapoptotic protein located in the ER and the nuclear membrane
J-C Bourdon1, J Renzing, P L Robertson
1Department of Surgery and Molecular Oncology, Ninewells Hospital and Medical School, Cancer Research Campaign (CRC) Cell Transformation Research Group, University of Dundee, Dundee DD1 9SY, Scotland, UK. j.bourdon@dundee.ac.uk
Abstract:
p53 is a transcription factor that induces growth arrest or apoptosis in response to cellular stress. To identify new p53-inducible proapoptotic genes, we compared, by differential display, the expression of genes in spleen or thymus of normal and p53 nullizygote mice after gamma-irradiation of whole animals. We report the identification and characterization of human and mouse Scotin homologues, a novel gene directly transactivated by p53. The Scotin protein is localized to the ER and the nuclear membrane. Scotin can induce apoptosis in a caspase-dependent manner. Inhibition of endogenous Scotin expression increases resistance to p53-dependent apoptosis induced by DNA damage, suggesting that Scotin plays a role in p53-dependent apoptosis. The discovery of Scotin brings to light a role of the ER in p53-dependent apoptosis.
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