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Related Experiment Videos

Selective stimulation of translational expression by Alu RNA.

Carol M Rubin1, Richard H Kimura, Carl W Schmid

  • 1Section of Molecular and Cellular Biology, University of California-Davis, Davis, CA 95616, USA.

Nucleic Acids Research
|July 24, 2002
PubMed
Summary

Human Alu and adenovirus VA1 RNAs selectively boost protein production without impacting global synthesis. This post-transcriptional effect, mediated by a PKR-independent pathway, offers insights into cell stress and viral infection recovery.

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Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • Short interspersed nuclear elements (SINEs) like Alu RNAs are abundant in eukaryotic genomes.
  • Adenovirus VA1 RNA is known to play a role in viral replication by modulating host cell translation.
  • The precise functions of many non-coding RNAs, including SINEs, remain incompletely understood.

Purpose of the Study:

  • To investigate the translational regulatory roles of human Alu RNAs and adenovirus VA1 RNA.
  • To elucidate the mechanism underlying the observed translational stimulation.
  • To explore potential roles of these RNAs in cellular processes like stress response and viral infection.

Main Methods:

  • Co-transient transfection assays using reporter genes in human and mouse cell lines.

Related Experiment Videos

  • Analysis of global protein synthesis rates and reporter mRNA abundance.
  • Mechanism studies involving PKR-independent pathways.
  • Deletion analysis of Alu RNA to identify active regions.
  • Testing of mouse B1 and B2 SINE RNAs.

    • Main Results:

    • Human Alu and adenovirus VA1 RNAs selectively enhanced reporter gene translational expression.
    • This stimulation occurred post-transcriptionally, without affecting global protein synthesis or mRNA levels.
    • The effect was transient, reducing the lag time for reporter gene expression.
    • A specific region within the right monomer of the Alu consensus sequence was essential for activity.
    • Mouse B1 and B2 SINE RNAs also stimulated reporter gene expression in mouse cells.

    Conclusions:

    • Alu and VA1 RNAs possess a novel mechanism for selectively enhancing protein translation.
    • This activity is PKR-independent and involves a specific structural element in Alu RNA.
    • The findings suggest potential roles for Alu and VA1 RNAs in cellular recovery from stress and during viral infections.
    • The translational stimulatory activity appears to be a general property of eukaryotic SINEs.