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Published on: May 13, 2014
Cloning of Human Glial Neurotrophic Factor and Its High Expression in Insect Cells
Zhe-Yu Chen1, Chang-Lin Lu, Jian-Xin Sun
1Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. xfwu@sunm.shcnc.ac.cn
Abstract:
The human glial cell derived neurotrophic factor (GDNF) was expressed in Tn-5B1-4 insect cells using the Bac to Bac baculovirus expression system, accounting for 30% of total cellular soluble proteins. The expressed product was purified by affinity chromatography and the activity assays showed that it could significantly promote the survival of dopaminergic neurons. The study can be the basis for further structural-functional analysis of GDNF.
