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Engineering Novel Functional Proteins Grafting Active Sites into Natural Scaffolds
Yu-Zhen Ye1, Hai-Xu Tang, Da-Fu Ding
1Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. dingdafu@server.shcnc.ac.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|July 24, 2002
Abstract:
Engineering novel small functional proteins by grafting active sites into small but stable proteins is an efficient protein design method. Combining heterogeneous self-consistent ensemble optimization (hetero-SCEO) with 3D-motif search tool, we developed a system to accomplish such method. It is tested by transferring zinc-binding site of carbonic anhydrase form B to charybdotoxin and its efficiency is demonstrated.