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New Regulatory Sequence of Human Connexin 26 Gene
Bai-Lu Peng1, Xun Zhang, Ming Zhou
1Cancer Research Institute, Hunan Medical University, Changsha 410078, China. ligy@public.cs.hn.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|July 24, 2002
Abstract:
Human connexin 26 gene (Cx26) has been considered a candidate suppressor gene in mammary epithelial cells. To explain regulatory mechanism of the Cx26, a DNase-1 hypersensitive 1.6 kb fragment upstream of the 5'-terminus of the gene was sequenced and assayed with CAT reporter system. The results showed that the 1.6 kb sequence had powerful promoter activity. The fragment contains two GT boxes (centering at -6158 and -6213 bp), and a TATA-less TTAAAA box (-6237/-6232 bp) which is the new promoter region of human Cx26.