LOOH, CD, TBARS and REM Kinetic Changes during Oxidation of High Density Lipoproteins Induced by Cu(2+) in Vitro

Oxidation of HDL can be mediated by Cu(2+) in vitro. In order to study kinetic changes of several products during HDL oxidatively modified in vitro, the kinetic changes of thiobarbituric acid reaction substance (TBARS), lipid hydroperoxide (LOOH), conjugated diene(CD) and relative electrophoretic mobility (REM) during the oxidation of HDL induced with Cu(2+) and HDL incubation 2-24 hours in vitro were observed. These results indicated that kinetic changes of LOOH and CD showed three different phases lag, propagation and decomposition phases and the kinetic changes of TBARS and REM showed two different phases lag and propagation phases, but no decomposition phase.