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Glucose and Insulin Regulate Leptin Expression in 3T3-F442A Adipocytes
Fang-Nian Wang1, Chun-Gu Ma, Nai-Xia Zhang
1The Laboratory of Molecular Genetics, Shanghai Medical University, Shanghai 200032, China. hysong@shmu.edu.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|July 24, 2002
Abstract:
Relative quantitation RT-PCR was used to investigate the regulation of leptin expression in 3T3-F442A adipocytes by glucose and insulin. The results showed that glucose and insulin stimulated the expression of leptin in 3T3-F442A adipocytes, but they did not act synergically. Over-high concentration of glucose suppressed the expression of leptin and the effect of insulin. The elevation of the expression of leptin was characterized with saturation. The concentration of glucose is very important for the regulation of leptin expression.