Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nitrite May Be an Important EDRF Modulator.

Ya-Ping Tian1, M. Rokicinski, W.H. Betts

  • 1Department of Clinical Biochemistry Chinese PLA General Hospital Beijing 100853 China. tianyp@plagh.com.cn

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|July 24, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional and clinical evidence for two novel heterozygous <i>BUB1B</i> variants and their value in precision genetic counseling for recurrent pregnancy loss.

Frontiers in endocrinology·2026
Same author

Case Report: Biallelic <i>PADI6</i> frameshift variants contribute to preimplantation embryonic lethality.

Frontiers in genetics·2026
Same author

Whole-genome sequencing identifies HOXD13 variants in syndactyly pedigrees.

Human genomics·2025
Same author

Deubiquitinase USP5 promotes acute myeloid leukemia through C2CD5 stabilization and PI3K/AKT/mTOR/HIF-1α-driven glycolysis.

Biochemical pharmacology·2025
Same author

Neonatal graves' disease complicated with cholestatic jaundice: a case report.

BMC endocrine disorders·2025
Same author

Adaptive Vectorial Restoration from Dynamic Speckle Patterns Through Biological Scattering Media Based on Deep Learning.

Sensors (Basel, Switzerland)·2025

Nitrite, not nitric oxide, may be the key endothelial-derived relaxing factor (EDRF). Low pH enables nitrite to form S-nitrosothiols, influencing thiol nitrosation in biological systems.

Area of Science:

  • Biochemistry
  • Physiology
  • Chemical Biology

Background:

  • Emerging evidence suggests S-nitrosothiols, rather than active nitric oxide, function as endothelial-derived relaxing factors (EDRFs).
  • At physiological pH (7.4), reduced thiols do not readily react with nitric oxide to form S-nitrosothiols; instead, they oxidize to stable nitrite.
  • Nitrite, however, reacts with reduced thiols at low pH to generate S-nitrosothiols.

Purpose of the Study:

  • To investigate the role of pH in the formation of S-nitrosothiols.
  • To explore the reactivity of nitrite and nitric oxide with thiols and hemoglobin at varying pH levels.
  • To propose a hypothesis regarding nitrite's function as an EDRF modulator.

Main Methods:

  • Comparative analysis of thiol-nitrite and thiol-nitric oxide reactions at physiological (pH 7.4) and low pH.

Related Experiment Videos

  • Examination of nitrite and nitric oxide interactions with deoxyhemoglobin and oxyhemoglobin across different pH conditions.
  • Assessment of pH influence on nitrosation processes.

    • Main Results:

    • Reduced thiols do not directly form S-nitrosothiols with nitric oxide at pH 7.4, yielding nitrite instead.
    • At low pH, nitrite rapidly reacts with reduced thiols to form S-nitrosothiols.
    • Both nitrite and nitric oxide react with deoxyhemoglobin and oxyhemoglobin at low pH, but not at pH 7.4, forming specific hemoglobin-nitrosyl complexes or oxidized states.

    Conclusions:

    • pH is a critical determinant of the nitrosation process, influenced by in vivo oxygen tension and redox status.
    • Nitrite's reactivity with thiols is highly pH-dependent.
    • A hypothesis is proposed where nitrite acts as an EDRF modulator, regulating thiol nitrosation in biological systems.