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Expression of Nerve Growth Factor from Agkistrodon halys Pallas in Silkworm Larvae
Li-Ying Guo1, Jie-Wu Liu, Xiang-Fu Wu
1Shanghai Institute of Biochemistry the Chinese Academy of Sciences Shanghai 200031 China. zhouyc@sunm.shcnc.ac.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|July 24, 2002
Abstract:
The cDNA encoding nerve growth factor (NGF) precursor from Agkistrodon halys Pallas (a Chinese snake species) was cloned into pBacPAK8 a baculovirus shuttle vector. The recombinant shuttle vector pBacPAK-NGF was coinfected with linear Bm-BacPAK6 DNA into BmN cells. The recombinant virus was screened and plaque-purified. The silkworm larvae were infected with the recombinant virus and collected 5 days later. The SDS-PAGE and the NGF activity by bioassay of PC12 cells have shown a high expression level of NGF of good biological activity in the larvae.