Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Validation of a 16-locus fluorescent multiplex system.

Benjamin E Krenke1, Allan Tereba, Stacey J Anderson

  • 1Promega Corporation, Madison, WI 53711-5399, USA.

Journal of Forensic Sciences
|July 26, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards a global DNA barcode reference library for quarantine identifications of lepidopteran stemborers, with an emphasis on sugarcane pests.

Scientific reports·2019
Same author

Developmental validation of the PowerPlex(®) Fusion 6C System.

Forensic science international. Genetics·2016
Same author

Advances in forensic DNA quantification: a review.

Electrophoresis·2014
Same author

Developmental validation of the PowerPlex(®) Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards.

Forensic science international. Genetics·2014
Same author

Developmental validation of the PowerPlex(®) 21 System.

Forensic science international. Genetics·2014
Same author

Developmental validation of the PowerPlex® Y23 System: a single multiplex Y-STR analysis system for casework and database samples.

Forensic science international. Genetics·2013

The PowerPlex 16 System offers efficient forensic DNA genotyping using single PCR amplification. Developmental validation confirmed its reliability across labs, though low DNA amounts can cause allele imbalance.

Area of Science:

  • Forensic Science
  • Genetics
  • Molecular Biology

Background:

  • Short tandem repeat (STR) multiplexes are crucial for forensic DNA profiling.
  • The PowerPlex 16 System includes core CODIS loci and additional markers for comprehensive genotyping.
  • Single PCR amplification enhances efficiency for forensic casework.

Purpose of the Study:

  • To conduct a developmental validation of the PowerPlex 16 System for forensic casework.
  • To assess laboratory-to-laboratory variation and ensure judicial acceptance.
  • To publish primer sequences and establish performance standards.

Main Methods:

  • Multi-laboratory developmental validation involving 24 laboratories.
  • Testing amplification reliability on various thermal cyclers and analysis platforms (ABI PRISM 310/377).

Related Experiment Videos

  • Evaluating genotyping consistency with varying DNA input amounts (0.0625-2 ng) and mixture analyses.

    • Main Results:

    • Reliable amplification and genotyping across different instruments and conditions.
    • Stochastic effects (allele imbalance) observed at low DNA concentrations (13% at 0.125 ng, 22% at 0.0625 ng).
    • Successful detection of alleles in mixtures up to 19:1, with robustness to moderate variations in reaction conditions.

    Conclusions:

    • The PowerPlex 16 System is a reliable and efficient tool for forensic DNA genotyping.
    • The system demonstrates consistent results compared to other commercial systems.
    • Environmental factors, particularly high temperatures, can impact genotype integrity.