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A method for the longitudinal sectioning of single hair samples.
Ivan M Kempson1, William M Skinner, Paul K Kirkbride
1Ian Wark Research Institute, University of South Australia, Mawson Lakes, Australia.
Journal of Forensic Sciences
|July 26, 2002
Summary
A new method allows longitudinal sectioning of single hair samples without embedding media. This technique reveals internal hair structure for analyzing xenobiotic distribution using surface analytical methods.
Area of Science:
- Forensic Science
- Analytical Chemistry
- Materials Science
Background:
- Analyzing xenobiotic distribution in hair requires precise sample preparation.
- Traditional methods often involve embedding media, complicating analysis.
Purpose of the Study:
- To present a simple, embedding-free technique for longitudinal sectioning of hair samples.
- To enable detailed analysis of internal xenobiotic distribution within hair shafts.
Main Methods:
- Hair samples are placed in a precisely grooved surface.
- Longitudinal sections are obtained via scraping or cutting.
- Sections are prepared for surface analytical techniques.
Main Results:
- Achieved planar longitudinal sections of single hair samples.
- Eliminated the need for embedding media during sectioning.
- Revealed internal hair structure for subsequent analysis.
Conclusions:
- The presented technique offers a straightforward approach to hair sample preparation.
- This method facilitates the study of xenobiotic distribution in both transverse and longitudinal planes.
- The technique is suitable for various surface analytical methods applied to hair analysis.