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Blood coagulation factor X (FX) in human seminal plasma
Y Matsuda1, K Shimokawa, M Katayama
1Meiji Pharmaceutical University, Kiyose-shi, Tokyo, Japan.
Archives of Andrology
|July 26, 2002
Summary
Active human blood coagulation factor X (FXa) was detected in semen. This enzyme, associated with FX, was identified using affinity chromatography and Western blot, with a molecular mass of 59 KDa.
Area of Science:
- Biochemistry
- Hematology
- Reproductive Biology
Background:
- Human blood coagulation involves a complex cascade of enzymes.
- Factor X (FX) is a key enzyme in the common pathway of coagulation.
- The presence and activity of FX in seminal plasma are not well-established.
Purpose of the Study:
- To detect and characterize the active form of human blood coagulation factor X (FXa) in human semen.
- To confirm the association of FX with seminal plasma.
- To determine the molecular mass of FX in semen.
Main Methods:
- Affinity chromatography using anti-human coagulation factor X antibodies.
- Enzyme activity assay using N-alpha-Benzoyl-L-isoleucyl-L-glutamyl-L-glycyl-L-arginine-p-nitroanilide (S-2222).
- Western blot analysis and SDS-PAGE to confirm identity and estimate molecular mass.
Main Results:
- Active human coagulation factor X (FXa) was detected in human semen (seminal plasma).
- The enzyme activity was inhibited by anti-human FX antibodies, confirming its identity.
- Western blot analysis confirmed the association of FX with seminal plasma.
- SDS-PAGE and Western blot estimated the molecular mass of mature FX to be 59 KDa.
Conclusions:
- Active FXa is present in human seminal plasma.
- FX in semen shares characteristics with blood coagulation FX.
- The molecular mass of FX in semen is approximately 59 KDa.