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Antioxidative collagen-derived peptides in human-placenta extract.
Shin-ichi Togashi1, Noriko Takahashi, Masanori Iwama
1Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan.
Placenta
|July 26, 2002
Summary
Human-placenta extract (PLx) contains potent antioxidant compounds. Researchers identified collagen-derived peptides as key contributors to PLx
Area of Science:
- Biochemistry
- Dermatology
- Cosmetology
Background:
- Human-placenta extract (PLx) is recognized for its significant antioxidant properties.
- PLx is extensively utilized in clinical and cosmetic applications.
- The specific antioxidative components within PLx require further elucidation.
Purpose of the Study:
- To identify and characterize the antioxidative components present in human-placenta extract (PLx).
- To investigate the chemical nature of novel antioxidants in PLx beyond previously known compounds.
Main Methods:
- Purification of PLx using Sephadex G-10 column chromatography.
- Further fractionation of active components via reverse-phase high-performance liquid chromatography (RP-HPLC).
- Analysis of antioxidant fractions using an amino acid sequencer.
Main Results:
- A fraction eluted from Sephadex G-10, devoid of known antioxidants (uracil, tyrosine, phenylalanine, tryptophan), retained significant antioxidant activity.
- RP-HPLC identified multiple peaks with antioxidant activity, all exhibiting optical density at 280nm.
- Amino acid sequencing revealed that six active fractions consisted of Glycine(G)-XY amino acid repeats, indicative of collagen-derived peptides.
Conclusions:
- Peptides derived from collagen are identified as novel antioxidative components of human-placenta extract.
- These collagen-derived peptides contribute significantly to the overall antioxidant capacity of PLx.
- The findings expand the understanding of bioactive compounds in PLx for potential therapeutic and cosmetic uses.