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Related Experiment Videos

Human sperm cells express CD44.

Rani Bains1, Jude Adeghe, Ray J Carson

  • 1Division of Biomedical Sciences, School of Health Sciences, University of Wolverhampton, Wolverhampton, United Kingdom.

Fertility and Sterility
|July 26, 2002
PubMed
Summary
This summary is machine-generated.

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This study confirms that CD44 is present on human sperm cells. Researchers used three methods to detect CD44 protein on sperm membranes, localizing it to the acrosome region.

Area of Science:

  • Reproductive biology
  • Cellular biology
  • Molecular biology

Background:

  • CD44 is a cell surface glycoprotein involved in cell-cell interactions.
  • Its role in human sperm function is not well understood.
  • Investigating CD44 expression on sperm may offer insights into male fertility.

Purpose of the Study:

  • To determine the expression of CD44 on human sperm cells.
  • To localize CD44 on sperm membranes.
  • To identify the molecular weight of CD44 in sperm.

Main Methods:

  • Prospective study involving 14 fertile donors.
  • Enzyme-linked immunoassay (ELISA) to measure CD44 concentration.
  • Immunocytochemistry and Western blotting to confirm and localize CD44 expression.

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Main Results:

  • CD44 was detected in pure sperm cell suspensions by ELISA.
  • Mean CD44 concentration was 0.013 ng per 10(6) sperm.
  • Immunocytochemistry confirmed CD44 on sperm membranes, primarily in the acrosome region.
  • Western blotting identified a 73 kDa CD44 variant.

Conclusions:

  • The expression of CD44 on human sperm membranes is confirmed.
  • Three independent methods validated the presence of CD44.
  • CD44 is localized to the acrosome region of human sperm.