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Published on: May 11, 2016
5Alpha-reductase type 2 genes in Japanese males do not appear to be associated with cryptorchidism
Yasuhiro Suzuki1, Isoji Sasagawa, Keiichi Itoh
1Department of Urology, Yamagata University School of Medicine, Yamagata, Japan.
Objective:
To evaluate the occurrence of mutations of 5alpha-reductase type 2 gene in patients with cryptorchidism or idiopathic azoospermia.
Design:
Controlled clinical study.
Setting:
Yamagata University Hospital, Yamagata and Tokyo Electric Power Company Hospital, Tokyo, Japan.
Patient(S):
Patients with isolated cryptorchidism (n = 48) or idiopathic azoospermia (n = 33) and a control group of males with proven fertility (n = 51).
Intervention(S):
Collection and analysis of blood, leukocytic DNA, semen, and testicular biopsy.
Main Outcome Measure(S):
Semen analysis, screening of the 5alpha-reductase type 2 gene within exon 1 using direct sequencing and exons 2-5 using single strand conformational polymorphism analysis.
Result(S):
No mutations were detected in isolated cryptorchid men, idiopathic azoospermic men, or controls within the 5 exons of the 5alpha-reductase type 2 gene.
Conclusion(S):
5Alpha-reductase type 2 gene abnormalities do not constitute a major factor in the development of cryptorchidism or idiopathic azoospermia.
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