Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Using inter-SINE-PCR to study mammalian phylogeny].

A A Bannikova1, V A Matveev, D A Kramerov

  • 1Department of Vertebrate Zoology, Lomonosov Moscow State University, Moscow, 119899 Russia.

Genetika
|July 26, 2002
PubMed
Summary

The inter-SINE-PCR method, using short interspersed elements, clarifies hedgehog family relationships but struggles with higher bat taxonomic levels. This DNA fingerprinting technique offers insights into mammalian evolution.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Participation of Proteins of the CPSF Complex in Polyadenylation of Transcripts Read by RNA Polymerase III from SINEs].

Molekuliarnaia biologiia·2024
Same author

Dark-Sector Search via Pion-Produced η and η^{'} Mesons Decaying Invisibly in the NA64h Detector.

Physical review letters·2024
Same author

[Regulation of Transcription by RNA Polymerase III Promotors in the Norm and Pathology].

Molekuliarnaia biologiia·2024
Same author

First Results in the Search for Dark Sectors at NA64 with the CERN SPS High Energy Muon Beam.

Physical review letters·2024
Same author

Search for Light Dark Matter with NA64 at CERN.

Physical review letters·2023
Same author

Multilocus Analysis of Phylogenetic Relationships in the Crocidura suaveolens Sensu Lato Species Complex: A Comparison with Mitochondrial Data.

Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections·2023

Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Genomics

Context:

  • Phylogenetic and taxonomic relationships in mammals are crucial for understanding evolutionary history.
  • Short interspersed elements (SINEs) are mobile genetic elements that can serve as markers for phylogenetic studies.
  • The inter-SINE-PCR technique amplifies DNA fragments located between SINE copies.

Purpose:

  • To evaluate the utility of the inter-SINE-PCR method for resolving phylogenetic and taxonomic relationships in mammals.
  • To investigate the evolutionary relationships within the families Vespertilionidae (bats) and Erinaceidae (hedgehogs).

Summary:

  • The study applied inter-SINE-PCR using mammalian interspersed repeat (MIR) and ERI-1 primers to analyze genetic divergence in bats and hedgehogs.
  • Results confirmed existing phylogenetic views for hedgehogs across genera Erinaceus, Hemiechinus, and Paraechinus, excluding Atelerix.

Related Experiment Videos

  • Phylogenetic reconstruction in bats yielded statistically significant results only at lower taxonomic levels, with higher ranks remaining unresolved.

    • Impact:

    • The inter-SINE-PCR method demonstrates effectiveness in resolving lower-level mammalian phylogenetic relationships.
    • The study highlights the limitations of inter-SINE-PCR for deep-level phylogenetic reconstructions in certain mammalian groups.
    • Findings contribute to a better understanding of mammalian evolutionary history and the application of molecular tools in taxonomy.