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Related Experiment Videos

Single-pulse nanoelectrospray ionization.

W Travis Berggren1, Michael S Westphall, Lloyd M Smith

  • 1Department of Chemistry, University of Wisconsin at Madison, 53706, USA.

Analytical Chemistry
|July 26, 2002
PubMed
Summary
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A novel electrospray ionization (ESI) source generates single ion packets for mass spectrometry. This new device offers attomole detection sensitivity and precise picoliter-level liquid handling for advanced analytical applications.

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Instrumentation

Background:

  • Conventional electrospray ionization (ESI) sources operate continuously, limiting control over ion generation and sample consumption.
  • Achieving high sensitivity in mass spectrometry often requires precise control over the sample introduction process.

Purpose of the Study:

  • To develop a new ESI source capable of generating discrete packets of ions for mass spectrometric analysis.
  • To enable attomole-level detection sensitivity through controlled sample dispensing.

Main Methods:

  • A piezoelectric element constricts a glass capillary (20-microm i.d.) to eject fixed volumes of sample solution.
  • The source dispenses volumes as small as 10 picoliters (pL).
  • Coupled with an orthogonal time-of-flight mass spectrometer for analysis.

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Main Results:

  • The new ESI source successfully generates single packets of ions.
  • Achieved attomole detection sensitivity with protein and DNA samples.
  • Demonstrated precise control over dispensed volume (10 pL), pulse frequency, and initiation/cessation of ionization.

Conclusions:

  • The developed ESI source provides picoliter-level control over sample dispensing and ionization pulsing.
  • This technology enhances detection sensitivity to the attomole range.
  • The source design is compatible with most ESI mass analyzers, offering broad applicability.