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Anaerobic Growth and Maintenance of Mammalian Cell Lines
07:15

Anaerobic Growth and Maintenance of Mammalian Cell Lines

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Storage of various cell lines at -70 degrees C or -80 degrees C in multi-well plates while attached to the substratum

Joe Corsini1, Francoise Maxwell, Ian H Maxwell

  • 1Biology Dept, Chadron State College, NE 69337, USA. jcorsini@csc.edu

Biotechniques
|July 26, 2002
PubMed
Abstract

No abstract available in PubMed .

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Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media