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Updated: Jun 23, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Published on: July 18, 2013
Two-hybrid systems and their usage in infection biology
Gouzel Karimova1, Daniel Ladant, Agnes Ullmann
1Unité de Biochimie Cellulaire, CNRS URA 2185, Institut Pasteur, Paris, France.
Abstract:
Two-hybrid systems are powerful tools to study protein-protein interactions in biological systems. The role of protein-protein interactions involved in pathogenesis of bacterial and viral infections were defined by using yeast or bacterial two-hybrid screens. Examples are given to highlight the specificity of interactions in signaling pathways, in regulation, secretion and structure-function relationships of virulence factors and their cellular targets. Two-hybrid systems were also used to establish large-scale protein interaction maps of viral and bacterial pathogens, that might be useful to identify targets for new drugs or vaccines.
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