Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fe(III)-mediated cellular toxicity.

Sangpen Chamnongpol1, Walter Dodson, Michael J Cromie

  • 1Department of Molecular Microbiology, Howard Hughes Medical Institute, Washington University School of Medicine, St Louis, MO 63110, USA.

Molecular Microbiology
|July 26, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short autoinhibitory sequences control phase separation of an essential bacterial transcription termination factor.

The EMBO journal·2026
Same author

A conserved peptidase governs glucose homeostasis in <i>Bacteroides</i>.

bioRxiv : the preprint server for biology·2026
Same author

The master virulence regulator PhoP dictates carbon metabolism by controlling cyclic AMP synthesis in Salmonella.

PLoS biology·2025
Same author

How Pathogens Maintain Proteostasis During Infection.

Molecular microbiology·2025
Same author

Hierarchical glycolytic pathways control the carbohydrate utilization regulator in human gut Bacteroides.

Nature communications·2025
Same author

Infection-relevant conditions dictate differential versus coordinate expression of <i>Salmonella</i> chaperones and cochaperones.

mBio·2025
Same journal

Riboflavin Salvage Supports Glycolysis in Borrelia burgdorferi Through Flavin-Dependent NAD<sup>+</sup> Regeneration.

Molecular microbiology·2026
Same journal

Distinct Spatial Organisation of Rho and RNA Polymerase in Salmonella Cells.

Molecular microbiology·2026
Same journal

A Single-Nucleotide Substitution Generates a de Novo Promoter That Activates a Latent Metabolic Bypass in Escherichia coli.

Molecular microbiology·2026
Same journal

A Phosphorylation-Dependent Partner-Switching-Like Module Regulates a Glycosyltransferase Required for Heterocyst Polysaccharide Layer Formation in Anabaena sp. Strain PCC 7120.

Molecular microbiology·2026
Same journal

Chain-Length Regulation by WzzE Is Necessary for, but Genetically Separable From, Cyclic Enterobacterial Common Antigen Synthesis.

Molecular microbiology·2026
Same journal

To Move or Not to Move: When and How Bacteria Suppress Flagellar Motility.

Molecular microbiology·2026
See all related articles

Iron(III) shows microbicidal activity against specific bacterial strains lacking the PmrA/PmrB system, independent of oxygen. This iron form damages bacteria differently than iron(II), impacting outer membrane permeability.

Area of Science:

  • Microbiology
  • Biochemistry
  • Toxicology

Background:

  • Iron is essential but can be toxic, with Fe(II) linked to free radical damage.
  • Fe(III) toxicity is less understood, particularly its role in bacterial defense mechanisms.

Purpose of the Study:

  • To investigate the microbicidal activity of Fe(III) against bacterial pathogens.
  • To elucidate the mechanism of Fe(III) toxicity in relation to iron regulatory systems.

Main Methods:

  • Testing Fe(III) microbicidal activity on Salmonella enterica, Escherichia coli, and Klebsiella pneumoniae strains.
  • Analyzing bacterial strains with defects in the PmrA/PmrB and Fur regulatory systems.
  • Assessing Fe(III) binding, outer membrane permeability, and vancomycin susceptibility.

Related Experiment Videos

Main Results:

  • Fe(III) exhibited microbicidal activity against bacteria deficient in the PmrA/PmrB system.
  • Fe(III) bound more effectively to pmrA mutants and acted independently of oxygen.
  • Fe(III) permeabilized the outer membrane of pmrA mutants, increasing vancomycin sensitivity.

Conclusions:

  • Fe(III) possesses oxygen-independent microbicidal activity distinct from Fe(II).
  • The PmrA/PmrB system is crucial for bacterial resistance to Fe(III) toxicity.
  • Fe(III) represents a potential antimicrobial agent targeting specific bacterial defense mechanisms.