Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Murine Nasal Lavage Fluid Collection without Blood Contamination05:12

Murine Nasal Lavage Fluid Collection without Blood Contamination

1.7K
The present protocol describes a novel method for avoiding blood contamination in murine nasal lavage fluid (NLF). Since this method ensures the NLF collection without blood contamination, it allows for more accurate detections of immunological components and various respiratory...
1.7K
Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome03:40

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome

1.1K
OSTSS leads to pooled inflammatory mediators in the tear film, causing symptoms like epiphora and discomfort. Here, we present a case where therapeutic nasolacrimal lavage every 2 months resolved epiphora and itchiness, suggesting its efficacy as a therapeutic intervention for OSTSS. Additionally, we report subjective improvements in symptoms in 3 additional...
1.1K
Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment04:35

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

880
Saline nasal lavage can be used to sample the canine nasal immune microenvironment. Because the approach is relatively non-invasive and does not disrupt the nasal tissues, it can be performed serially. Cells and proteins collected from the nasal lavage technique can be processed for various laboratory analyses.
880
Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber08:47

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

2.9K
A protocol for conducting a challenge in an allergen exposure chamber (AEC) facility is presented. AECs have proven to be safe and effective tools for the induction of allergic symptoms or as an endpoint in the efficacy testing of allergen immunotherapy due to their ability to maintain stable particle concentrations and environmental...
2.9K
Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians06:49

Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians

12.0K
Batrachochytrium dendrobatidis infection in amphibians can be eliminated using a reduced concentration (0.0025%) and shorter duration (five minute baths for six days) of itraconazole than is generally used. Less mortality and fewer negative side effects were observed using this modified treatment...
12.0K
Intranasal Administration of CNS Therapeutics to Awake Mice07:15

Intranasal Administration of CNS Therapeutics to Awake Mice

61.4K
A method to intranasally administer drugs to awake mice for the purpose of targeting the brain is described. This method allows for repeat dosing over long periods using intranasal administration of drug without anesthesia, and nose-to-brain delivery with minimal systemic...
61.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Platinum thin film-based matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry imaging for improved ion yields of adrenocortical steroids.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2026
Same author

The C-terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice.

FEBS open bio·2026
Same author

The atypical E3 ligase HOIL-1 safeguards the ribosome during cellular stress.

Nature cell biology·2026
Same author

LRBA organizes distinct vesicular trafficking systems in distal nephron segments for water and sodium conservation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Investigation of the mechanisms underlying the development and evolution of the mammalian cerebrum using gyrencephalic ferrets.

Frontiers in cell and developmental biology·2026
Same author

Asymmetry in ubiquitin binding by A20 reveals early recognition features of the ubiquitin code.

FEBS letters·2026
Same journal

Daily briefing: How cooperation built the world.

Nature·2026
Same journal

Deep-sea oddities and boatloads of other new species - June's best science images.

Nature·2026
Same journal

From cloning to gene-editing: the enduring legacy of Dolly the sheep.

Nature·2026
Same journal

Time to give hydration breaks the red card? What science says about keeping cool.

Nature·2026
Same journal

Universities are relying on AI-detection software to catch cheating. How well do the programs work?

Nature·2026
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
See all related articles

Related Experiment Video

Updated: Jan 8, 2026

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome
03:40

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome

Published on: April 25, 2025

1.1K

E3 ubiquitin ligase that recognizes sugar chains.

Yukiko Yoshida1, Tomoki Chiba, Fuminori Tokunaga

  • 1Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613, Japan.

Nature
|July 26, 2002
PubMed
Summary
This summary is machine-generated.

N-glycans signal protein degradation via the SCF(Fbx2) ubiquitin ligase complex. This mechanism targets N-glycosylated proteins translocated from the endoplasmic reticulum (ER) for degradation, crucial for protein quality control.

More Related Videos

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment
04:35

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

Published on: December 27, 2024

880
Intranasal Administration of CNS Therapeutics to Awake Mice
07:15

Intranasal Administration of CNS Therapeutics to Awake Mice

Published on: April 8, 2013

61.4K

Related Experiment Videos

Last Updated: Jan 8, 2026

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome
03:40

Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome

Published on: April 25, 2025

1.1K
Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment
04:35

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

Published on: December 27, 2024

880
Intranasal Administration of CNS Therapeutics to Awake Mice
07:15

Intranasal Administration of CNS Therapeutics to Awake Mice

Published on: April 8, 2013

61.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • N-glycosylation in the endoplasmic reticulum (ER) is vital for protein quality control.
  • Protein degradation pathways ensure cellular proteostasis.

Purpose of the Study:

  • To investigate the role of N-glycans in protein degradation.
  • To identify the specific ubiquitin ligase complex involved in N-glycan-mediated degradation.

Main Methods:

  • Studied the interaction between the F-box protein Fbx2 and N-glycosylated proteins.
  • Utilized a mutant Fbx2 lacking the F-box domain (Fbx2 Delta F) to assess its impact on degradation pathways.
  • Investigated protein interactions following proteasome inhibition.

Main Results:

  • N-glycans act as degradation signals recognized by the SCF(Fbx2) ubiquitin ligase complex.
  • Fbx2 specifically binds to proteins with N-linked high-mannose oligosaccharides.
  • Pre-integrin beta 1 interacts with Fbx2 in the cytosol.
  • Mutant Fbx2 Delta F inhibits the degradation of ER-associated degradation substrates.

Conclusions:

  • The SCF(Fbx2) complex ubiquitinates N-glycosylated proteins.
  • This ubiquitination occurs after translocation from the ER to the cytosol, mediated by the protein quality control mechanism.