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Twist functions in mouse development.

Meredith P O'Rourke1, Patrick P L Tam

  • 1Embryology Unit, Children's Medical Research Institute, University of Sydney, Wentworthville, NSW, Australia.

The International Journal of Developmental Biology
|July 27, 2002
PubMed
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The transcription factor Twist plays a crucial role in the development of limbs and craniofacial structures. Loss of Twist function in mouse embryos disrupts mesodermal differentiation, limb patterning, and neural crest cell migration.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Limb and craniofacial development share common morphogenetic mechanisms.
  • Genetic activity profiles and developmental defects suggest shared pathways.
  • The transcription factor Twist is implicated in these developmental processes.

Purpose of the Study:

  • To review findings on molecular pathways disrupted by Twist loss-of-function.
  • To highlight the role of Twist in mesodermal differentiation, limb and branchial arch development, and neural crest cell migration.
  • To investigate the differential requirement of Twist in forelimb versus hindlimb development and its interaction with Gli3.

Main Methods:

  • Analysis of Twist mutant mouse embryos.
  • Genetic, functional, and microarray analyses to identify downstream targets and interacting genes.

Related Experiment Videos

  • Review of recent studies on Twist function.

    • Main Results:

    • Twist has an evolutionarily conserved function in mesodermal differentiation.
    • Loss of Twist function affects limb and branchial arch outgrowth and patterning, and neural crest cell migration.
    • Twist shows differential requirements in forelimb vs. hindlimb development and interacts with Gli3 for anterior digit formation.

    Conclusions:

    • Twist is essential for coordinating development of multiple body parts, including limbs and craniofacial structures.
    • The findings reveal conserved functions of Twist across species.
    • Twist's role in mesodermal differentiation and cell migration is critical for normal development.