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Related Experiment Videos

IgE-reactive proteins from Stachybotrys chartarum.

Charles Barnes1, Steve Buckley, Freddy Pacheco

  • 1Section of Allergy/Immunology, The Children's Mercy Hospital, Kansas City, Missouri 64108, USA.

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
|July 27, 2002
PubMed
Summary
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Sensitivity to Stachybotrys chartarum (black mold) is more common than thought, with nearly half the population showing IgG and almost 10% showing IgE antibodies. This fungus may significantly impact allergic and asthmatic individuals.

Area of Science:

  • Environmental microbiology
  • Immunology
  • Mycology

Background:

  • Stachybotrys chartarum (black mold) is linked to infant pulmonary hemorrhage and increasingly reported in homes of patients with allergies and asthma.
  • The mycotoxins produced by this fungus are suspected to be the cause of these health issues.

Purpose of the Study:

  • To evaluate the antigenic proteins of Stachybotrys chartarum.
  • To determine the prevalence of immunoglobulin (Ig)E and IgG antibodies against these proteins in the general population.

Main Methods:

  • Stachybotrys chartarum was cultured, and extracts were prepared from spores and mycelia.
  • Plasma from 132 healthy individuals was tested for IgG and IgE against S. chartarum using enzyme immunoassays.
  • IgE-binding proteins were identified and characterized by molecular weight using SDS-PAGE immunoblotting.

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Main Results:

  • 49.2% of sera tested had IgG antibodies against S. chartarum.
  • 9.4% of sera tested had IgE antibodies against S. chartarum.
  • Two IgE-binding proteins of 34 kDa and 52 kDa were identified in S. chartarum extracts.

Conclusions:

  • Sensitivity to Stachybotrys chartarum may be more widespread than previously recognized.
  • The fungus poses a potential risk to individuals with asthma and allergies through immunologic and toxic mechanisms.
  • The role of Stachybotrys chartarum among allergenic fungi warrants re-evaluation.