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Updated: Sep 30, 2026

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
Published on: October 1, 2009
Purification and characterization of a new lectin from the red marine alga Hypnea musciformis
C S Nagano1, F B M B Moreno, C Bloch
1Laboratório de Bioquímica Marinha - BioMol-Lab, Depto de Engenharia de Pesca, Universidade Federal do Ceará, Caixa Postal 6033, CEP 60451-970, Brasil.
Abstract:
A lectin from the red marine alga Hypnea musciformis (HML) was purified by extraction with 20 mM PBS, precipitation with 70% saturated ammonium sulphate, ion-exchange DEAE-Cellulose chromatography and RP-HPLC. The 9.3 kDa polypeptide agglutinates erythrocytes from various sources and shows oligomerization tendencies under certain MALDI-TOF/MS conditions. Preliminary N-terminal sequencing and biological assays strongly suggest that the HML may belong to a new class of algae lectins.
