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Published on: May 29, 2021
Topographical analysis of Schizolobium parahyba chymotrypsin inhibitor (SPCI) by atomic force microscopy
José Roberto S A Leite1, Luciano Paulino Silva, Clarice Cunha Taveira
1Laboratório de Morfologia e Morfogênese, Departamento de Genética e Morfologia, Universidade de Brasília, DF, Brazil.
Abstract:
Atomic Force Microscopy (AFM) has been a useful tool for molecular surface analysis and to estimate topographical properties of proteins. Here we report a topographical study of a chymotrypsin inhibitor from Schizolobium parahyba seeds (SPCI) by AFM. The underlying structure of SPCI oligomers has been resolved in nanometer order resolution. SPCI oligomerize in hexagonal, ellipsoid, comet, pyramidal, and "Z" shaped. The hexagonal was the most observed oligomer shape.
