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Cloning and expression of human beta-defensin-2 gene in Escherichia coli
Xiangming Fang1, Li Peng, Zhinan Xu
1The Central Laboratory of Sir Run Run Shaw Hospital, School of Medical Science, Zhejiang University, Hangzhou 310027, The People's Republic of China.
Protein and Peptide Letters
|July 27, 2002
Abstract:
Human beta-defensin-2 (hBD-2), a small cationic peptide, exhibits a broad range of antimicrobial activity. It has been found to play important roles in innate and adaptive immune responses against microbial invasion. For the purposes of this study, hBD-2 gene was cloned from the lesions of human condyloma acuminatum. An expression vector was constructed and transformed into E. coli. hBD-2 was expressed as a fusion protein in both the soluble and insoluble forms, which was further confirmed by western blotting analysis.