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Published on: October 18, 2017
Immobilized earthworm fibrinolytic enzyme III-1 with carbonyldiimidazole activated-agarose
Xue-Qin Wu1, Cen Wu, Rong-qiao He
1Lab of Visual Information Processing, Academic Center of Brain and cognition, Institute of Biophysics, The Chinese Academy of Sciences, 15 Da Tun Rd, Beijing 100101, China.
Abstract:
Earthworm fibrinolytic enzyme III-1 (EFE-III-1) was prepared to couple with cross-linking agarose activated by 1,'-Carbonyl- diimidazole (CDI) in this study. Although the activity of the immobilized protease decreased to approximately 64% of the native enzyme, the activity of EFE-III-1 coupled with the resin activated by CDI was higher than that activated by cyanogen bromide (CNBr). The immobilized protease was experimentally demonstrated to hydrolyze IgG, albumin and creatine kinase, besides fibrin(ogen) and plasmin(ogen), suggesting that EFE had a broad substrate specificity.
