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Related Experiment Videos

The methods to generate transgenic animals and to control transgene expression.

Louis-Marie Houdebine1

  • 1Biologie du Développement et Biotechnologies, Institut National de la Recherche Agronomique, 78352 Jouy en JosasCedex, France. houdebine@diamant.jouy.inra.fr

Journal of Biotechnology
|July 27, 2002
PubMed
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Transgenic animals offer advanced models for studying gene function and human diseases. New techniques enhance gene editing and expression, paving the way for improved animal production and pharmaceutical development.

Area of Science:

  • Biotechnology
  • Genetics
  • Animal Science

Background:

  • Transgenic animals are established tools for gene function studies and disease modeling.
  • Genome sequencing advancements have further validated the use of transgenic models.
  • The potential for transgenic animals as bioreactors for pharmaceuticals is emerging.

Purpose of the Study:

  • To review recent advancements in animal transgenesis techniques.
  • To highlight improvements in generating transgenic models for research and production.
  • To discuss emerging tools for gene manipulation and expression control.

Main Methods:

  • Utilizing transposons, retroviruses, and sperm-mediated gene transfer for transgenic generation.
  • Employing somatic cell nuclear transfer with gene-edited cells.

Related Experiment Videos

  • Applying the Cre-LoxP system for targeted genomic modification.
  • Leveraging improved tetracycline systems for controlled transgene expression.
  • Developing RNA-based tools (siRNA, shRNA, ribozymes) and dominant-negative proteins for gene inhibition.

    • Main Results:

    • Easier and more accessible generation of transgenic animals across various species.
    • Enhanced precision in gene targeting and modification using systems like Cre-LoxP.
    • Improved control over transgene expression through refined inducible systems.
    • Development of novel tools for specific gene silencing in host or viral contexts.
    • Increased feasibility of precise allele replacement in livestock for breeding improvement.

    Conclusions:

    • Recent technical innovations have significantly advanced animal transgenesis.
    • These advancements provide more precise models for gene function and disease research.
    • Transgenesis holds promise for revolutionizing pharmaceutical production and animal agriculture.