Suppressive effects of tea polyphenol and conformational changes with receptor for advanced glycation end products
Moriatsu Takada1, Yonson Ku, Hirochika Toyama
1First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. moriatsu@med.kobe-u.ac.jp
Background/Aims:
It is reported that polyphenol is associated with low risk of hepatoma and that RAGE (receptor for advanced glycation end products) is important for cancer invasion.
Methodology:
Effects of teapolyphenol, EGCG (epigallocatechin-3-gallate) were studied. Proliferation of on human hepatoma cells, HLF, was measured with the use of WST-1 colorimetric assay. Cell invasion was analyzed by the Matrigel invasion assay. Morphology and immunohistological staining of expression of RAGE were also performed.
Results:
Proliferation was inhibited with the addition of EGCG in a dose-dependent manner. EGCG (200 mumol/L) produced a profound growth suppression of HLF cells (24.5%). Cell invasion was also inhibited with preincubation of 100 mumol/L of EGCG (10.2%). In addition to the antitumor effects, neurite-like conformational changes of HLF cells were observed. Addition of EGCG (100 mumol/L) showed the expression of RAGE on cell surface in accordance to the morphological changes.
Conclusions:
The pathway associated to cell movement might be activated with RAGE expression. Although EGCG inhibits the growth and invasion, the cells which expressed RAGE seem to survive. Thus, the enrollment of RAGE should be analyzed to clarify the mechanisms of cancer resistance.
Insights
Epigallocatechin-3-gallate (EGCG), a polyphenol, inhibits hepatoma cell growth and invasion. However, EGCG also induces the expression of receptor for advanced glycation end products (RAGE), suggesting a role in cancer resistance.
Area of Science:
- Hepatocellular carcinoma research
- Cancer biology
- Molecular oncology
Background:
- Polyphenols are linked to reduced hepatoma risk.
- Receptor for advanced glycation end products (RAGE) is implicated in cancer invasion.
Purpose of the Study:
- Investigate the effects of tea polyphenol, epigallocatechin-3-gallate (EGCG), on human hepatoma cells (HLF).
- Examine EGCG's impact on cell proliferation, invasion, and RAGE expression.
Main Methods:
- WST-1 colorimetric assay for cell proliferation.
- Matrigel invasion assay for cell invasion analysis.
- Immunohistological staining for RAGE expression and morphological observation.
Main Results:
- EGCG significantly inhibited HLF cell proliferation and invasion in a dose-dependent manner.
- EGCG treatment induced neurite-like morphological changes and increased RAGE expression on HLF cells.
- Observed growth suppression of 24.5% at 200 µmol/L EGCG and 10.2% invasion inhibition at 100 µmol/L EGCG.
Conclusions:
- EGCG exhibits potent anti-proliferative and anti-invasive effects on hepatoma cells.
- RAGE expression may be linked to cell movement pathways and potentially cancer cell survival.
- Further analysis of RAGE is crucial for understanding hepatoma resistance mechanisms.
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