[A new strategy for detecting Lac I mutants based on pMCLac I/neo transgenic mice]

We studied a novel mutation detection method of the two Lac I target genes in pMCLac I/neo transgenic mice. The transgenic mice that contain two types of Lac I genes in pMCLac I/neo vector are different from the transgenic mice carrying only one target gene. Therefore a novel method to detect mutation quickly and efficiently has become a new project after the establishment of pMCLac I/neo transgenic mice. In this paper, a positive selection system--M9/L is used. The result showed that M9/L positive selection system was able to detect the two Lac I target genes, suggesting that it is a rapid and effective method to detect the target mutations in the pMCLac I/neo transgenic mouse.