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Related Experiment Videos

Allergens in celery and zucchini.

Stefan Vieths1, D Lüttkopf, J Reindl

  • 1Department of Allergology, Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany.

Allergy
|July 30, 2002
PubMed
Summary

This study confirms celery and zucchini allergies using double-blind placebo-controlled food challenges (DBPCFC). Ubiquitous cross-reactive structures like Bet v 1 and cross-reactive carbohydrate epitopes (CCD) are key allergens in celery and zucchini allergy.

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Area of Science:

  • Allergology
  • Food Science
  • Immunology

Background:

  • Celery and zucchini allergies are increasingly recognized but allergen identification remains crucial.
  • Accurate diagnosis requires validated challenge procedures and molecular allergen characterization.

Purpose of the Study:

  • To confirm celery and zucchini allergies using double-blind placebo-controlled food challenges (DBPCFC).
  • To identify specific allergens in celery and zucchini recognized by patient IgE antibodies.
  • To investigate the role of cross-reactive structures and pollen sensitization.

Main Methods:

  • Developed a safe DBPCFC protocol involving spit and swallow methods for celery and zucchini.
  • Utilized immunoblot inhibition assays with allergen extracts, recombinant allergens, and N-glycans.

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  • Analyzed patient IgE binding to identify specific allergens and cross-reactive epitopes.

    • Main Results:

    • Celery allergy confirmed in 69% of subjects; zucchini allergy in 4 subjects.
    • Systemic reactions occurred in 50% of celery-allergic and 75% of zucchini-allergic patients during DBPCFC.
    • Bet v 1-related allergens, cross-reactive carbohydrate epitopes (CCD), and profilin were identified as major allergens in celery and zucchini.

    Conclusions:

    • Ubiquitous cross-reactive structures are significant in both celery and zucchini allergies.
    • Celery allergy shows a specific association with birch pollen allergy (Api g 1), unlike zucchini allergy.
    • Validated DBPCFC and molecular diagnostics are essential for accurate allergy diagnosis and management.