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Binding of FGF-1 variants to protein kinase CK2 correlates with mitogenicity

Camilla Skiple Skjerpen1, Trine Nilsen, Jørgen Wesche

  • 1Department of Biochemistry at The Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway.

The EMBO Journal
|July 30, 2002
PubMed

Insights

Fibroblast growth factor-1 (FGF-1) binds to protein kinase CK2 (CK2) within cells. This interaction may influence FGF-1

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Fibroblast growth factor-1 (FGF-1) exhibits both extracellular and intracellular functions.
  • Identifying intracellular binding partners is crucial for understanding FGF-1's diverse roles.
  • Protein kinase CK2 (CK2) is a key regulator of various cellular processes.

Purpose of the Study:

  • To identify intracellular binding partners of FGF-1.
  • To investigate the interaction between FGF-1 and protein kinase CK2 (CK2).
  • To explore the functional implications of the FGF-1/CK2 interaction in cellular processes.

Main Methods:

  • Protein isolation from U2OS human osteosarcoma cells using FGF-1 as bait.
  • Surface plasmon resonance (SPR) to quantify binding kinetics (K(D) values).
  • A novel in vivo assay to confirm intracellular protein interactions.
  • In vitro phosphorylation assays and autophosphorylation studies.

Main Results:

  • Protein kinase CK2 (CK2) was identified as an intracellular binding partner for FGF-1.
  • FGF-1 binds to both the alpha and beta subunits of CK2 with measured affinities.
  • FGF-1 and FGF-2 are phosphorylated by CK2 in vitro, and their presence enhances CK2 beta autophosphorylation.
  • A correlation exists between the mitogenic potential of FGF-1 mutants and their binding affinity to CK2 alpha.

Conclusions:

  • FGF-1 directly interacts with protein kinase CK2 (CK2) intracellularly.
  • CK2 may play a role in mediating FGF-1's intracellular functions, including mitogenesis.
  • Further research is warranted to elucidate the precise mechanisms of CK2 in FGF signaling pathways.

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