Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microarray analysis of gene expression in the aging human retina.

Shigeo Yoshida1, Beverly M Yashar, Suja Hiriyanna

  • 1Department of Ophthalmology and Visual Sciences, W. K. Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan 48105, USA.

Investigative Ophthalmology & Visual Science
|July 31, 2002
PubMed
Summary

Human retinal gene expression changes with age, impacting energy metabolism and stress response pathways. This study identifies candidate genes for age-related retinal diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conserved host genes act as anchors for fast-evolving piRNA clusters via promoter hijacking.

Research square·2026
Same author

Stress Responsive bZIP Transcription Factors ATF4 and BACH1 Cooperate With MAF-Family bZIP Protein NRL to Fine-Tune Rod Photoreceptor Gene Expression.

Investigative ophthalmology & visual science·2026
Same author

Revisiting retinal and macular degeneration in the genomics era.

Nature reviews. Genetics·2026
Same author

Halofantrine protects photoreceptors in multiple models of retinal degeneration.

Research square·2026
Same author

To refer or not to refer? Exploring the cognitive process of genetic counselors' decision to refer a patient to another professional.

Journal of genetic counseling·2026
Same author

Asparagus racemosus Root Extract (SheVari4®) Alleviates Menopausal Symptoms in Pre-, Peri-, and Post-menopausal Healthy Women.

Cureus·2026

Area of Science:

  • Genomics
  • Ophthalmology
  • Molecular Biology

Background:

  • Human retinal aging is a complex process.
  • Understanding age-related changes in retinal gene expression is crucial for identifying disease mechanisms.
  • Previous studies have not comprehensively profiled gene expression in aging human retinas.

Purpose of the Study:

  • To create gene expression profiles for young and elderly human retinas.
  • To pinpoint candidate genes involved in aging-associated retinal diseases.
  • To investigate the role of specific gene pathways in retinal aging.

Main Methods:

  • Utilized gene microarray slides with 2400 human genes.
  • Hybridized labeled target cDNAs synthesized from young and elderly human retinal RNAs.

Related Experiment Videos

  • Validated microarray findings using Northern blot analysis and quantitative reverse transcription PCR (qRT-PCR).

    • Main Results:

    • Over 50% of genes on the microarray hybridized to retinal cDNA targets.
    • Detected significant expression changes in 24 genes between young and elderly retinas.
    • Confirmed expression changes in 8 out of 10 validated genes, involving energy metabolism, stress response, cell growth, and neuronal signaling.

    Conclusions:

    • Human retinal aging is associated with altered gene expression patterns.
    • Stress response and energy metabolism pathways are critical in retinal aging.
    • Gene microarrays are effective for global retinal gene expression analysis, aiding in the study of diseases like age-related macular degeneration.