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[Expression of adenovirus-mediated pGH cDNA with first intron in CHO cells]
Xiu-Jin Li1, Fei Zhong, Xin-Zhong Wang
1Hebei Vocational and Technical Teachers College, Changli 066600, China.
Abstract:
The recombinant adenoviruses containing pGH cDNA and pGH cDNA with the first intron under the control of CMV promoter were constructed respectively by homogenous recombination method. The results showed that the recombinant adenoviruses could mediate pGH cDNA expression in CHO cells infected with the recombinant adenoviruses. The expression level of pGH cDNA with the first intron increased by 117% compared with pGH cDNA without intron. This indicate that the first intron of pGH gene have the function of improving the expression of the pGH gene.