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Preferential expression of ADP-ribosylation factor gene in the chick embryonic gonads
Masa-aki Hattori1, Tsuyoshi Fujioka, Yukio Kato
1Division of Animal and Marine Resource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka, Japan. mhattori@agr.kyushu-u.ac.jp
Abstract:
cDNA cloning from chick embryonic gonads subtracted from tissues of the brain, heart, liver, gizzard, mesonephros and skeletal muscle was performed to identify genes with expression unique to embryonic gonads. Several cDNA clones encoding characterized as well as many uncharacterized genes were obtained. ADP-ribosylation factor (ARF) of these identified genes was preferentially expressed in the chick embryonic ovary and testis as revealed by reverse transcription-polymerase chain reaction analysis. Expression of the ARF was evaluated through embryonic development, but no difference in the transcript (relative to glyceraldehyde-3-phosphate dehydrogenase transcript) was observed between the left and right ovaries, and between the ovary and testis. In addition, the ARF transcript was detected in the gonads on embryonic days 5 to 21. These findings indicate that the ARF is constantly, but preferentially expressed in the embryonic gonads during development.