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Cellular uptake of N-methylpyrrole/N-methylimidazole polyamide-dye conjugates
Jason M Belitsky1, Stephanie J Leslie, Paramjit S Arora
1Division of Chemistry and Chemical Engineering and Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA.
Bioorganic & Medicinal Chemistry
|August 2, 2002
Abstract:
The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide-dye conjugates in a variety of living cells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide-dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.