Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyelectrolyte-coated ion exchangers for cell-resistant expanded bed adsorption.

Maria B Dainiak1, Igor Yu Galaev, Bo Mattiasson

  • 1Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. 124, S-221 00 Lund, Sweden.

Biotechnology Progress
|August 3, 2002
PubMed
Summary

Modified anion exchangers with poly(acrylic acid) (PAA) prevent yeast cell adhesion during expanded bed adsorption. This innovation enables efficient protein purification from complex fermentation broths, improving downstream processing.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent Advances in Optical Sensing for the Detection of Microbial Contaminants.

Micromachines·2023
Same author

Preparation of Staphylococcal Protein A Imprinted Supermacroporous Cryogel Beads.

Methods in molecular biology (Clifton, N.J.)·2022
Same author

Upgrading of bio-separation and bioanalysis using synthetic polymers: Molecularly imprinted polymers (MIPs), cryogels, stimuli-responsive polymers.

Engineering in life sciences·2022
Same author

A Sensitive Capacitive Biosensor for Protein a Detection Using Human IgG Immobilized on an Electrode Using Layer-by-Layer Applied Gold Nanoparticles.

Sensors (Basel, Switzerland)·2022
Same author

Evaluation of Polytyramine Film and 6-Mercaptohexanol Self-Assembled Monolayers as the Immobilization Layers for a Capacitive DNA Sensor Chip: A Comparison.

Sensors (Basel, Switzerland)·2021
Same author

Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes.

Turkish journal of chemistry·2021

Area of Science:

  • Bioseparation Engineering
  • Protein Purification
  • Chromatography

Background:

  • Expanded bed adsorption chromatography is crucial for direct protein capture from fermentation broths.
  • Biomass contamination of adsorbents is a significant limitation, hindering direct application.
  • Existing STREAMLINE DEAE anion exchangers exhibit strong interactions with cells and debris.

Purpose of the Study:

  • To develop a cell-resistant anion exchanger for expanded bed adsorption.
  • To prevent biomass contamination during protein purification from fermentation broths.
  • To maintain the binding capacity of the adsorbent for target proteins.

Main Methods:

  • Modification of STREAMLINE DEAE anion exchanger beads with a poly(acrylic acid) (PAA) shielding layer via electrostatic interactions.

Related Experiment Videos

  • Utilizing high-degree polymerization PAA to ensure surface adsorption at pore mouths.
  • Testing the modified adsorbent's interaction with yeast cells and its capacity for bovine serum albumin (BSA) purification.

    • Main Results:

    • PAA coating effectively prevented yeast cell adhesion to the anion exchanger beads.
    • The PAA layer minimally impacted the binding capacity for bovine serum albumin (BSA).
    • The modified adsorbent successfully isolated BSA from a mixture containing BSA, lysozyme, and yeast cells.

    Conclusions:

    • Poly(acrylic acid)-coated STREAMLINE DEAE offers a cell-resistant solution for expanded bed adsorption.
    • This modification enhances the applicability of direct capture chromatography in bioprocessing.
    • The PAA coating is stable under mild elution conditions, allowing for repeated purification cycles.