Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ouabain-binding protein(s) from human plasma.

Behnaz Parhami-Seren1, Richard Haberly, Michael N Margolies

  • 1Department of Surgery, Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Mass 02129, USA.

Hypertension (Dallas, Tex. : 1979)
|August 3, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The root of this evil: microangiopathic hemolytic anemia and renal failure.

The American journal of medicine·2014
Same author

Measurement of glomerular filtration rate in anesthetized and conscious rhesus monkeys (Macaca mulatta).

American journal of veterinary research·2010
Same author

The "normal" factor VIII concentration in plasma.

Thrombosis research·2010
Same author

Quantitation of anti-factor VIII antibodies in human plasma.

Blood·2009
Same author

Scarce among men.

The American journal of medicine·2007
Same author

Endogenous ouabain: upregulation of steroidogenic genes in hypertensive hypothalamus but not adrenal.

Circulation·2005
Same journal

Brain Iron in Nucleus Accumbens and Cognitive Function in Preeclampsia.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

Hypertension Treatment Intentions: A Health Belief Model Analysis.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

Changes in Retinal Microvasculature During Healthy Pregnancy Measured by AO.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

Longitudinal Maternal IgG and IgA Glycosylation Profiles in Pregnancy Reveal Early Immune Alterations in Placenta-Related Complications: The Rotterdam Periconception Cohort.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

Blood Pressure Variability and Outcomes Across Antihypertensive Regimens.

Hypertension (Dallas, Tex. : 1979)·2026
Same journal

Rural Health and Health Disparities in Hypertension Management: A Scientific Statement From the American Heart Association.

Hypertension (Dallas, Tex. : 1979)·2026
See all related articles

Researchers identified a ouabain-like compound bound to human immunoglobulins in plasma. These immunoglobulin complexes may act as a carrier system for regulating sodium-potassium ATPase activity in the body.

Area of Science:

  • Biochemistry
  • Physiology
  • Immunology

Background:

  • Mammalian Na+,K+-ATPase is crucial for cellular function and shares a binding site with cardiac glycosides.
  • A search for endogenous mammalian analogs of these plant compounds is ongoing.
  • The circulating form of the ouabain-like compound, a potential endogenous analog, is largely unknown.

Purpose of the Study:

  • To investigate the circulating form of the ouabain-like compound in human plasma.
  • To identify potential carrier proteins for the ouabain-like compound.
  • To characterize the interaction between the ouabain-like compound and its carrier proteins.

Main Methods:

  • Production of a ouabain-specific monoclonal antibody (mAb 1-10).
  • Probing normal human plasma for ouabain-protein complexes using mAb 1-10.

Related Experiment Videos

  • Isolation and purification of protein-bound ouabain-like activity using techniques like Protein A and C18 chromatography.
  • Assay of Na+ pump activity in human erythrocytes.

    • Main Results:

    • Ouabain-like biological activity was found associated with 80, 50, and 25 kDa proteins, identified as human immunoglobulin or immunoglobulin-like molecules.
    • These immunoglobulin-like proteins specifically bound ouabain (or its isomer) with high affinity, but not digoxin.
    • The purified fractions inhibited Na+,K+-ATPase activity in human erythrocytes, suggesting physiological relevance.

    Conclusions:

    • Human immunoglobulins or immunoglobulin-like molecules may serve as a carrier system for ouabain-like compounds in circulation.
    • These complexes could function as a reservoir and delivery system for modulating Na+, K+-ATPase activity in vivo.
    • This finding provides insight into the endogenous regulation of the sodium-potassium pump.