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Updated: Aug 10, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments
Published on: April 20, 2012
Ultrasonic wave properties in the particle compounded agarose gels
Mami Matsukawa1, Tadaaki Akimoto, Shizuko Ueba
1Faculty of Engineering, Doshisha University, Kyotanabe-shi, Kyoto, Japan. mmatsuka@mail.doshisha.ac.jp
Abstract:
Ultrasonic wave properties (attenuation and velocity) in the particle compounded agarose gels have been experimentally studied in the range from 1 to 30 MHz. The particles used were talc, glass beads and graphite. The effects of size and volume concentration of particles were clearly observed as changes of ultrasonic wave properties. Applying the Urick's theory for viscous liquid suspensions, the specific curves of velocity in the gels were observed as a function of a beta, where a is the radius of the particles and beta is described by angular frequency omega, density rho and fluid viscosity eta. This indicates that the particle behavior in the gels seems to be similar with that in the viscous fluid. The estimated eta in the gels was higher than that of the free water, showing the high viscosity in the gels.
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