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Tnfrsf13c (Baffr) is mis-expressed in tumors with murine leukemia virus insertions at Lvis22
Kathryn E Hentges1, Sujatha P Yarlagadda, Monica J Justice
1Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
Abstract:
In susceptible strains of mice, leukemia is caused by the somatic integration of murine leukemia retroviruses into the host genome. Integration sites that are common to several tumors are likely to affect genes that are important in oncogenesis. Here we present the analysis of a common site of retroviral integration on mouse chromosome 15, which includes the genomic structure of three genes near the integration site. One of the genes misexpressed at the insertion site has recently been characterized as a B-cell receptor, Tnfrsf13c (formerly Baffr), indicating that this approach is useful in defining genes that function in lymphocyte development and tumor progression. Current genome databases provide powerful resources for the rapid identification of genes at common proviral insertion sites. The characterization of these genes in tumor samples will allow a function to be assigned to many novel loci identified by the genome sequencing projects.
Insights
Murine leukemia retroviruses cause cancer by integrating into mouse DNA. Researchers identified a common integration site on chromosome 15, revealing a gene crucial for B-cell development and tumor progression.
Area of Science:
- Oncology
- Genomics
- Immunology
Background:
- Murine leukemia retroviruses induce leukemia via somatic integration into the host genome.
- Common retroviral integration sites suggest disruption of critical oncogenesis genes.
- Identifying these genes aids understanding of lymphocyte development and cancer progression.
Purpose of the Study:
- To analyze a common retroviral integration site on mouse chromosome 15.
- To identify and characterize genes affected by retroviral insertion.
- To explore the role of these genes in oncogenesis and lymphocyte development.
Main Methods:
- Analysis of common retroviral integration sites in murine leukemia.
- Genomic structure analysis of genes near the integration site on chromosome 15.
- Utilizing current genome databases for gene identification.
Main Results:
- A common retroviral integration site was identified on mouse chromosome 15.
- Genomic analysis revealed three genes near the integration site.
- One affected gene, Tnfrsf13c (Baffr), is a B-cell receptor involved in lymphocyte development.
Conclusions:
- Retroviral integration site analysis is effective for identifying genes in oncogenesis.
- Tnfrsf13c (Baffr) misexpression at the integration site impacts lymphocyte development and tumor progression.
- Genome databases facilitate rapid identification of novel genes at proviral insertion sites, aiding functional assignment.