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A quantitative and validated SAGE transcriptome reference for adult mouse heart.

Sergey V Anisimov1, Kirill V Tarasov, Michael D Stern

  • 1Laboratory of Cardiovascular Science, National Institute on Aging, NIH Baltimore, Maryland 21224, USA.

Genomics
|August 6, 2002
PubMed
Summary

This study presents the first quantitative gene expression profile of adult mouse hearts using Serial Analysis of Gene Expression (SAGE). The findings establish a reliable cardiac transcriptome reference for identifying dynamic gene expression changes.

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Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Genomics

Background:

  • Transcriptome analysis is crucial for understanding gene function in development, aging, and disease.
  • Accurate quantification of gene expression is essential for reliable biological interpretation.

Purpose of the Study:

  • To quantify the functionally active genome of adult C57BL/6 mouse heart (AMH).
  • To establish a reliable transcriptome reference for cardiac gene expression studies.

Main Methods:

  • Serial Analysis of Gene Expression (SAGE) was employed to sequence 88,860 tags from AMH.
  • Quantitative PCR was used for independent validation of transcript abundance.

Main Results:

  • Over 66% of unique tags matched known genes or expressed sequence tags (ESTs).

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  • Mitochondrial transcripts comprised 18.7% and sarcomeric proteins 3.2% of total transcripts.
  • SAGE data revealed quantitative discrepancies compared to cDNA arrays, with PCR confirming SAGE reliability.

    • Conclusions:

    • Serial Analysis of Gene Expression provides a quantitatively reliable method for transcriptome profiling.
    • This study presents the first quantitative expression profile of the adult mouse heart transcriptome.
    • The established cardiac transcriptome serves as a vital reference for future studies on dynamic gene expression changes.