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Lens epithelial cell death after cataract surgery.

Shizuya Saika1, Takeshi Miyamoto, Iku Ishida

  • 1Department of Ophthalmology, Wakayama Medical University, 811-1 Kimidera, Wakayama 641-0012, Japan. shizuya@wakayama-med.ac.jp

Journal of Cataract and Refractive Surgery
|August 6, 2002
PubMed
Summary

Lens epithelial cells undergo apoptosis after cataract surgery, particularly in the early healing stages. Transforming growth factor-beta2 may induce this cell death in the lens capsule.

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Regenerative Medicine

Background:

  • Cataract surgery involves removing the clouded lens.
  • The healing process of the lens capsule after surgery is not fully understood.
  • Lens epithelial cells (LECs) play a role in wound healing.

Purpose of the Study:

  • To investigate if lens epithelial cells undergo apoptosis during the healing of the postoperative lens capsule.
  • To further characterize the cellular mechanisms involved in lens capsule healing after cataract surgery.

Main Methods:

  • Detection of apoptotic cells in human postoperative lens capsules using the TUNEL assay.
  • Investigation of the effect of transforming growth factor-beta2 (TGF-beta2) on mouse LEC apoptosis in an organ-culture system.

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Main Results:

  • TUNEL-positive cells, indicative of apoptosis, were found in 3 out of 17 human postoperative specimens.
  • Apoptotic LECs were predominantly observed in early-stage (<10 days) healing beneath the anterior capsule.
  • TGF-beta2 treatment induced apoptosis in mouse LECs in an organ-culture model.

Conclusions:

  • Lens epithelial cells undergo apoptosis during the healing process following cataract surgery, especially in the early postoperative period.
  • Transforming growth factor-beta2 is a potential inducer of apoptosis in LECs in vivo.