Dynamics of length-force relations in airway smooth muscle

We tested the plasticity hypothesis that isometric force could optimize to similar levels, independent of muscle length after repeated contractions in bovine airway smooth muscle. In constant length experiments in which muscle lengths were held constant, we found that total force remained significantly length-dependent during repeated contractions, and changed by tenfold between 40 and 100% optimal length (L(o)). Passive force contributed to < 10% of total force. In sequential length experiments, total force increased by fourfold between 38 and 75% L(o), but changed insignificantly between 75 and 100% L(o), suggesting limited force plasticity near L(o). Force became attenuated after each length change, but remained length-dependent during redevelopment. Changing length from L(o) to 150% L(o) induced proportional decrease in active force and increase in passive force, with insignificant change in total force. Furthermore, post-stretch force redeveloped at L(o) was substantially attenuated, resulting in the shifting of the length-total force relation toward longer length. The observed complex dynamics of length-force relations could explain the complex lung mechanics in vivo.