Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altered urinary bladder function in mice lacking the vanilloid receptor TRPV1.

L A Birder1, Y Nakamura, S Kiss

  • 1Department of Medicine, Laboratory of Epithelial Cell Biology, Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA. lbirder@pitt.edu

Nature Neuroscience
|August 6, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting neurotrophin and nitric oxide signaling to promote recovery and ameliorate neurogenic bladder dysfunction following spinal cord injury - Mechanistic concepts and clinical implications.

Continence (Amsterdam, Netherlands)·2023
Same author

Soluble Guanylate Cyclase Activators to Treat Benign Prostatic Hyperplasia and associated LUTS.

Continence (Amsterdam, Netherlands)·2023
Same author

Serotonergic paraneurones in the female mouse urethral epithelium and their potential role in peripheral sensory information processing.

Acta physiologica (Oxford, England)·2017
Same author

Modulation of spontaneous activity in the overactive bladder: the role of P2Y agonists.

American journal of physiology. Renal physiology·2012
Same author

Is the urothelium intelligent?

Neurourology and urodynamics·2010
Same author

Animal models and their use in understanding lower urinary tract dysfunction.

Neurourology and urodynamics·2010

The transient receptor potential vanilloid 1 (TRPV1) channel is crucial for normal bladder function. Mice lacking TRPV1 show altered bladder contractions and impaired purinergic signaling in urothelial cells.

Area of Science:

  • Urology
  • Neuroscience
  • Cell Biology

Background:

  • The capsaicin-gated ion channel TRPV1 is present in both nerve terminals and epithelial cells of the urinary bladder.
  • Its precise role in bladder function and urothelial signaling remains to be fully elucidated.

Purpose of the Study:

  • To investigate the significance of TRPV1 expression in bladder function and purinergic signaling.
  • To analyze bladder function in mice genetically deficient in TRPV1.

Main Methods:

  • Analysis of bladder function in TRPV1 knockout (trpv1(-/-)) mice and wild-type littermates.
  • In vivo assessment of bladder contractions and spinal cord signaling under anesthesia.
  • In vitro studies of ATP release and membrane capacitance in excised bladders and cultured urothelial cells.

Related Experiment Videos

Main Results:

  • TRPV1-deficient mice exhibited increased frequency of low-amplitude, non-voiding bladder contractions.
  • Reduced spinal cord signaling and reflex voiding were observed in trpv1(-/-) mice during bladder filling.
  • Mechanically evoked (stretch) and osmotically evoked ATP release from urothelial cells were significantly diminished in the absence of TRPV1.

Conclusions:

  • TRPV1 plays a significant role in maintaining normal urinary bladder function.
  • TRPV1 is essential for mechanically evoked purinergic signaling by the urothelium.
  • These findings highlight TRPV1 as a key mediator of bladder mechanosensation and signaling.