Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A PCR-based amplification method retaining the quantitative difference between two complex genomes.

G Mike Makrigiorgos1, Subrata Chakrabarti, Yuzhi Zhang

  • 1Department of Radiation Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA. mmakrigiorgos@partners.org

Nature Biotechnology
|August 6, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Drug-resistant abdominal tuberculosis in children: dilemmas and difficulties.

Infectious diseases (London, England)·2026
Same author

MEDUSA: Maintaining Entire DNA Duplexes for Utmost Sequencing Accuracy.

Clinical chemistry·2026
Same author

Differential epigenetic regulation of glucose-induced alteration of miR-9 in retinal and cardiac endothelial cells.

PloS one·2026
Same author

JP2 and JCN Crosstalk Abrogate MURF1-Mediated JCN Ubiquitination and Degradation in Cardiomyocytes.

JACC. Basic to translational science·2026
Same author

Silencing of lncRNA HOTAIR Using Eyedrops as a Potential Treatment for Diabetes-Associated Retinal Dysregulation and Dysfunction.

Investigative ophthalmology & visual science·2026
Same author

Assessment of the Correlation between Macroscopic ICRS Grading and Histopathological OARSI Scoring in Osteoarthritic Cartilage: An <i>Ex-Vivo</i> Analysis.

Cartilage·2026
Same journal

Unlocking the chemical potential of filamentous fungi using prime editing.

Nature biotechnology·2026
Same journal

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
Same journal

Prime editing for precise genome engineering and modulation of fungal metabolism.

Nature biotechnology·2026
Same journal

Retargeted serine integrases for one-step, precise integration of large DNA sequences in human cells.

Nature biotechnology·2026
Same journal

A retargeted recombinase for precise insertion of large DNA.

Nature biotechnology·2026
Same journal

Experiment-guided AlphaFold3 resolves measurement-consistent protein ensembles.

Nature biotechnology·2026
See all related articles

Balanced PCR offers a novel method for accurately comparing DNA samples by amplifying them together, overcoming PCR

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Genome-wide analysis technologies require sufficient DNA material for comparative studies.
  • Standard Polymerase Chain Reaction (PCR) amplification is nonlinear and prone to impurities, affecting quantitative accuracy.
  • Separate amplification of DNA samples distorts the original quantitative relationships between genes.

Purpose of the Study:

  • To introduce a novel 'balanced PCR' procedure for faithful quantitative comparison of two complex DNA samples.
  • To overcome the limitations of nonlinear amplification and impurity influence in standard PCR.

Main Methods:

  • Two distinct genomic DNA samples are tagged with unique and common DNA sequences.
  • Pooled DNA samples are amplified in a single PCR reaction using the common tag.

Related Experiment Videos

  • Amplified pooled samples are subsequently separated using the unique DNA tags.

    • Main Results:

    • Balanced PCR accurately retains the quantitative differences between amplified genes.
    • The method eliminates the influence of PCR bias and impurities by pooling samples.
    • Validation demonstrated successful application with synthetic DNA, genomic DNA, and cDNA on microarrays.

    Conclusions:

    • Balanced PCR enables unbiased amplification of allelic fragments from complex DNA mixtures, even after multiple PCR rounds.
    • This technique facilitates genetic analysis in challenging samples like minute tissues or single cells.
    • The method significantly improves the reliability of comparative genomic analyses.