Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD5- mantle cell lymphoma.

Zach Liu1, Henry Y Dong, Wojciech Gorczyca

  • 1IMPATH, New York, NY 10019, USA.

American Journal of Clinical Pathology
|August 7, 2002
PubMed
Summary

Mantle cell lymphoma (MCL) can lack CD5 expression, challenging diagnosis. Evaluating bcl-1 protein is crucial for identifying these CD5-negative MCL cases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Viral dynamics of acute SARS-CoV-2 infection and applications to diagnostic and public health strategies.

PLoS biology·2021
Same author

Performance of the 4Kscore Test in Plasma and Serum and Stability of the Component Analytes in Clinical Samples.

The journal of applied laboratory medicine·2021
Same author

Cyclin D1 + large B-cell lymphoma with altered CCND1 and BCL-6 rearrangements: a diagnostic challenge.

Biomarker research·2019
Same author

Acute myeloid leukemia in a father and son with a germline mutation of <i>ASXL1</i>.

Biomarker research·2018
Same author

p53 status in the primary tumor predicts efficacy of subsequent abiraterone and enzalutamide in castration-resistant prostate cancer.

Prostate cancer and prostatic diseases·2018
Same author

A case of lenalidomide-dependent myelodysplastic syndrome.

Blood advances·2018

Area of Science:

  • Hematology
  • Oncology
  • Pathology

Background:

  • Mantle cell lymphoma (MCL) is typically characterized by B-cell antigen and CD5 expression, along with bcl-1 protein overexpression.
  • Unusual presentations of bcl-1 positive and CD5 negative MCL pose diagnostic and management difficulties.

Purpose of the Study:

  • To investigate cases of CD5-negative lymphoma with features consistent with MCL.
  • To determine the diagnostic utility of bcl-1 expression in the absence of CD5.

Main Methods:

  • Analysis of 25 cases (48 samples) of bcl-1 positive and CD5 negative lymphoma.
  • Assessment of CD5 expression using flow cytometry and immunohistochemistry.
  • Morphological evaluation for MCL features (centrocytic and blastic variants).
  • Cytogenetic analysis including fluorescence in situ hybridization (FISH) for t(11;14) translocation.

Related Experiment Videos

Main Results:

  • Twenty-five cases of bcl-1 positive, CD5 negative lymphoma were identified.
  • Morphological features consistent with MCL were observed in 20 cases (centrocytic) and 5 cases (blastic variant).
  • The characteristic t(11;14) translocation was confirmed in 8 of 11 cases by FISH and in 2 additional cases by cytogenetics.

Conclusions:

  • Mantle cell lymphoma can present without CD5 expression.
  • Immunohistochemical evaluation of bcl-1 or molecular genetic testing is essential for diagnosing MCL when CD5 is negative but clinical or morphological suspicion remains.