Related Experiment Videos
Design of buffer exchange surfaces and sensor chips for biosensor chip mass spectrometry
Dobrin Nedelkov1, Kemmons A Tubbs, Randall W Nelson
1Intrinsic Bioprobes Inc., Tempe, AZ 85281, USA. dnedelkov@intrinsicbio.com
Proteomics
|August 8, 2002
Summary
This study demonstrates buffer exchange for biosensor chip mass spectrometry. New base sensor chips were constructed and functionalized for improved analyte detection and analysis using matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Materials Science
Background:
- Biosensor chip mass spectrometry requires efficient analyte handling and detection.
- Developing novel sensor chip architectures and chemistries is crucial for advancing analytical techniques.
- Existing methods may face limitations in analyte elution and verification.
Purpose of the Study:
- To demonstrate the feasibility of buffer exchange in biosensor chip mass spectrometry.
- To construct and characterize novel base sensor chips with alternative surface chemistries.
- To verify analyte presence using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry.
Main Methods:
- Immobilization of anti-beta-2-microglobulin (beta2m) antibody on a sensor chip for analyte capture.
- Utilizing low and high pH buffers for analyte elution and routing to a cation exchanger flow cell.
- Fabrication of gold-sputtered glass slides with self-assembled monolayers for chip activation.
- Derivatization of flow cells with antibodies for beta-2-microglobulin (beta2m) and cystatin C (cysC).
- Analysis using surface plasmon resonance (SPR) and MALDI-TOF mass spectrometry.
Main Results:
- Successful demonstration of buffer exchange for analyte manipulation within biosensor flow cells.
- Construction of functionalized base sensor chips capable of capturing and retaining specific analytes.
- Verification of beta-2-microglobulin (beta2m) and cystatin C (cysC) presence in respective flow cells via MALDI-TOF MS.
- Absence of artifacts from the employed surface chemistries in the mass spectrometry analysis.
Conclusions:
- Buffer exchange is a feasible technique for biosensor chip mass spectrometry.
- Novel base sensor chips with alternative chemistries can be effectively constructed and utilized.
- The developed methods allow for specific analyte capture, elution, and subsequent mass spectrometry verification without significant artifacts.