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Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions
Published on: September 10, 2021
[Enteropathogenic agents isolated in persistent diarrhoea]
D López Marín1, E Sagaró Gonzáles, M Valdés Depena
1Especialista de Primer Grado de Pediatría, Hospital Juan Manuel Márquez, Perú.
Insights
Salmonella was the most common bacterial cause of diarrhea in children. Shigella was more frequent in persistent diarrhea cases, while Cryptosporidium and Giardia were equally prevalent parasites.
Area of Science:
- Pediatrics
- Infectious Diseases
- Microbiology
Background:
- Diarrhea remains a significant health concern in pediatric populations.
- Understanding the causative agents of acute and persistent diarrhea is crucial for effective treatment.
Purpose of the Study:
- To identify the most frequent bacterial and parasitic pathogens in children with acute and persistent diarrhea.
- To compare the prevalence of different pathogens between acute and persistent diarrhea cases.
Main Methods:
- Retrospective review of clinical records of 4445 children diagnosed with diarrhea between January 1990 and December 1993.
- Analysis of bacteriological and parasitological results from stool samples of patients with confirmed infections.
Main Results:
- Salmonella species were the most frequently isolated bacteria in both acute and persistent diarrhea groups.
- Shigella demonstrated a higher incidence in persistent diarrhea cases compared to acute diarrhea.
- Enteroaggregative E. coli (EAEC) and Enterohemorrhagic E. coli (EHEC) showed similar distribution patterns.
- Cryptosporidium and Giardia were the most common parasitic findings, with similar prevalence.
Conclusions:
- Salmonella is a primary bacterial pathogen causing childhood diarrhea in this cohort.
- Shigella plays a more significant role in persistent diarrhea.
- Parasitic infections by Cryptosporidium and Giardia are common and equally distributed.
Abstract:
Out of 45558 patients admited in our hospital, from January 1990 to December 1993, the clinical records of 4445 children with diarrhoea (4289 with acute diarrihoea and 156 with persistent diarrhoea) were reviewed. Those with positive bacteriological or parasitological results were taken as sample. Salmonella was the bacteria most frequently isolated in both groups of patients. ECEP and ECEH had a similar distribution while Shigella showed a higher frequency in cases of persistent diarrhoea, for a third pace of incidence. From the parasitological point of view, Cryptosporidium and Giardia had the same behavior.
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