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Related Experiment Videos

Esterase activity in various Candida species.

E Aktas1, N Yigit, A Ayyildiz

  • 1Department of Microbiology, Faculty of Medicine, Atatürk University, Erzurum, Turkey. aeaktas@hotmail.com

The Journal of International Medical Research
|August 9, 2002
PubMed
Summary
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This study investigated esterase activity in Candida species using the Tween 80 opacity test. Most Candida albicans, Candida tropicalis, and Candida guilliermondii strains showed positive esterase activity, indicating the test

Area of Science:

  • Microbiology
  • Medical Mycology

Background:

  • Candida species are opportunistic fungal pathogens.
  • Esterase activity is a potential virulence factor and diagnostic marker in Candida.

Purpose of the Study:

  • To determine the esterase activity profiles of various Candida species.
  • To evaluate the utility of the Tween 80 opacity test for assessing esterase activity in Candida.

Main Methods:

  • Isolation and identification of 125 Candida strains using conventional methods.
  • Assay of esterase activity via the Tween 80 opacity test.

Main Results:

  • High prevalence of positive esterase activity observed in Candida albicans (58/59 strains), Candida tropicalis (38/38 strains), and Candida guilliermondii (3/3 strains).

Related Experiment Videos

  • Other Candida species tested did not exhibit significant esterase activity.
  • The Tween 80 opacity test proved effective for detecting esterase activity.

    • Conclusions:

    • Candida albicans, Candida tropicalis, and Candida guilliermondii are significant producers of esterases.
    • The Tween 80 opacity test is a reliable, cost-effective, and user-friendly method for assessing Candida esterase activity.